2016.3.21纤维素降解菌和蛋白质降解菌多样性的研究.pdf

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Jan. 2009, p. 286–291 Vol. 75, No. 1 0099-2240/09/$08.00 0 doi:10.1128/AEM.00607-08 Copyright © 2009, American Society for Microbiology. All Rights Reserved. Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia)† Ilya V. Kublanov,1,4* Anna A. Perevalova,1 Galina B. Slobodkina,1 Aleksander V. Lebedinsky,1 Salima K. Bidzhieva,1 Tatyana V. Kolganova,2 Elena N. Kaliberda,3 Lev D. Rumsh,3 4 1 Thomas Haertle´, and Elizaveta A. Bonch-Osmolovskaya Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia1 ; Bioengineering Center, Russian Academy of Sciences, Moscow, Russia2; Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia3 4 ; and L’Institut National de la Recherche Agronomique, Nantes, France Received 13 March 2008/Accepted 22 October 2008 Samples of water from the hot springs of Uzon Caldera with temperatures from 68 to 87°C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or - or -keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several