湿害对不同大麦基因型抗氧化酶系统影响.pdf

浙江大学学报( ) 35( 3) : 315~ 320, 2009 Journal of Zhejiang University ( Agric Life Sci) :2009) DOI: 10.3785/ j. issn. 2009. 03.013 1 1 1, 2 张晓勤 , 吴克利, 薛大伟 ( 1. , 310036; 2. , 310029) : 对耐湿性不同的2 个大麦品种Yerong( 耐性较强) 和Franklin( 耐性较弱) 在苗期进行田间湿 试验, 研究二者叶片中抗氧化酶活性及丙二醛(M DA) 含量的变化. 结果表明, 湿 改变了植株叶片 MDA 含量和超氧化物歧化酶( SOD) 过氧化物酶( POD) 和过氧化氢酶( CAT) 活性. 总体上, SOD 和 POD 活性在湿 胁迫早期均升高,但随着湿 时间的延长, Franklin 叶片的SOD 活性开始下降. 湿 下 CAT 活性变化在2 个大麦品种中不一致. 2 种大麦叶片中MDA 含量均在湿 后呈上升趋势, 而且 Franklin 比Yerong 积累更多的 MDA,表明随着湿 胁迫时间延长, Franklin 细胞膜伤 比 Yerong 严 重. 可见,湿 逆境下叶片较高的 SOD 活性和较低的MDA 含量与Yerong 耐湿性较强有关; 在 一定程 度上M DA 含量高低SOD 和POD 活性可以作为大麦耐湿性强弱的生理指标. : 湿 ; 大麦; 叶片; 抗氧化酶; 丙二醛 : S5123; S42 : A 1 1 1, 2 ZHA G Xiaoqin , WU Keli , XU E Dawei ( 1. College of L if e and Env ironment S ciences, H angz hou Normal University, H angz hou 31 36, China; 2 . Dep artment of A gronomy , College of A griculture Biotechnolog y, Zhej iang Univ ers ity , H angz hou 31 29, China) Effects of waterlogging stress on antioxidative enzyme system in different barley genotypes Journal of Zhejiang University ( Agric Life Sci) , 2009,35( 3) :315320 Abstract: Antioxidant enzyme activities and malondialdehyde ( MDA) content of two barley cultivars, Yerong ( waterloggingtolerant ) and Franklin ( waterloggingsensitive) were studied with a field experiment under control and waterlogging stress. Waterlogging stress altered MDA content and the activities of superoxide dismutase ( SOD) , peroxidase ( POD) and catalase ( CAT) in barley leaves. In general, increase of SOD and POD induced by waterlogging were found at early stage of waterlogging stress, how