二龄草鱼脾脏肝脏组织高表达甘露糖结合凝集素mRNA-CURRENT.PDF

动物学报 　50 ( 1) : 137 - 140 , 2004 A cta Zoologica S inica 　　　　　 　 二龄草鱼脾脏、肝脏组织高表达甘露糖 结合凝集素 mRNA 吴　超　陆承平 南京农业大学动物医学院 , 南京 210095 The mannosebinding lectin mRNA is expressed at high level in spleen and liver in adult grass carp WU Chao , L U ChengPing College of Veterinary Medicine , Nanjin g Agriclture University , Nanjin g 　210095 , China Abstract 　Innate immunity is expected to be very important in fish. Mannosebingding lectin (MBL) participates in the innate immune system as an activator of the complement system and as an opsonin after binding to certain carbohydrate structures on microorganisms. In this experiment , total mRNA was isolated from spleen , liver , gills , thymus , head kid ney and kidney of adult and immature grass carp Ctenophary godon idllus . The cDNA of MBL was obtained by RTPCR using total mRNA from the spleen of carp as template. Such cDNA was labled with 32 P and used as probe for Northern analysis , and autoradiographic signals were quantified by densitometry analysis. The results showed that MBL was high expressed in the spleen and liver and low in gills , thymus , head kidney and kidney of adult grass carp , and MBL was much lower expressed in spleen and liver of immature grass carp than those of adult grass carp. The results might partially explain why immature grass carp are vulnerable to grass carp hemorrhage virus ( GCHV) whereas adult grass carp are not. This suggested that MBL may be an important antiGCHV factor [ Acta Zoologica Sinica 50 ( 1) : 137 - 140 , 2004 ]. Key words 　Grass carp , Ctenophary godon idllus , Mannosebinding lectin , Northern blot , Densitometry analysis 关键词 　草鱼 　甘露糖结合凝集素 　Northern 杂交 　密度测定分析 　　甘露糖结合凝集素 ( Mannosebinding lectin , 理后易被补体杀灭 , 但