色谱柱和色谱系统的故障检修解析.pptVIP

1 HPLC Column and System Troubleshooting 色谱柱和色谱系统的故障检修 What Every HPLC User Should Know 每个HPLC使用者应该知道什么 HPLC Components HPLC 的组成 Pump 泵 Injector/Autosampler 进样器/自动进样器 Column 色谱柱 Detector 检测器 Data System/Integrator 数据系统/积分仪表 All of these components can have problems and require troubleshooting. 所有这些部件可能出现故障而需要检修。 Categories of Column Problems 色谱柱问题的类型 A. Pressure 压力 B. Peak shape 峰形 C. Retention 保留时间 Pressure Issues压力问题 Column Observations Potential Problems 色谱柱观测 潜在的问题 High pressure Plugged frit 压力高 堵塞滤头 Column contamination 色谱柱污染 Piugged packing 堵塞填料 Determining the Cause and Correcting High Back Pressure 查出原因 纠正压力 Check pressure with/without column - many pressure problems are due to blockages in the system or guard 有／无柱子时检查压力 － 许多压力问题是由于系统或保护柱的阻塞 If Column Pressure is high 如果柱压高: Wash column - Eliminate column contamination and 冲洗柱子 plugged packing 排除柱污染和填料堵塞 - high molecular weight/adsorbed compounds 高分子量／被吸附化合物 - precipitate from sample or buffer 样品或缓冲液中的沉淀物 Back flush column - Clear plugged frit 反冲柱子－清洁阻塞的滤头 Change frit - Clear plugged frit 更换滤头 Column Cleaning 柱子清洗 F lush with stronger solvents than your mobile phase 用比你的流动相更强的溶剂冲洗 Reversed-Phase Solvent Choices in Order of Increasing Strength 为了增加强度选择反相溶剂 Use at least 25 ml of each solvent for analytical columns 对于分析柱每种溶剂至少用25 ml 冲洗 Mobile phase without buffer salts 没有缓冲盐的流动相 100% Methanol 甲醇 100% Acetonitrile 乙腈 75% Acetonitrile :25% Isopropanol 75乙腈 ： 25％异丙醇 100% Isopropanol 异丙醇 100% Methylene Chloride★ 二氯甲烷 100% Hexane ★ 已烷 ★ When using