2nd international external quality control assessment for the molecular diagnosis of dengue infections第二国际的外部质量控制评估的分子诊断登革热感染.pdfVIP

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2nd international external quality control assessment for the molecular diagnosis of dengue infections第二国际的外部质量控制评估的分子诊断登革热感染.pdf

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2nd international external quality control assessment for the molecular diagnosis of dengue infections第二国际的外部质量控制评估的分子诊断登革热感染

2nd International External Quality Control Assessment for the Molecular Diagnosis of Dengue Infections 1 1 1 1 1 Cristina Domingo , Matthias Niedrig , Anette Teichmann , Marco Kaiser , Leonid Rumer , Richard G. 2 1 Jarman , Oliver Donoso-Mantke * 1 Robert Koch Institute, Berlin, Germany, 2 United States Army Medical Component of the Armed Forces Research Institute of the Medical Sciences (USAMC-AFRIMS), Bangkok, Thailand Abstract Background: Currently dengue viruses (DENV) pose an increasing threat to over 2.5 billion people in over 100 tropical and sub-tropical countries worldwide. International air travel is facilitating rapid global movement of DENV, increasing the risk of severe dengue epidemics by introducing different serotypes. Accurate diagnosis is critical for early initiation of preventive measures. Different reverse transcriptase PCR (RT-PCR) methods are available, which should be evaluated and standardized. Epidemiological and laboratory-based surveillance is required to monitor and guide dengue prevention and control programmes, i.e., by mosquito control or possible vaccination (as soon as an effective and safe vaccine becomes available). Objective: The purpose of the external quality assurance (EQA) study described is to assess the efficiency and accuracy of dengue molecular diagnosis methods applied by expert laboratories. Study Design: A panel of 12 human plasma samples was distributed and tested for DENV-specific RNA. The panel comprised 9 samples spiked with different DENV serotypes (DENV-1 to DENV-4), including 10-fold dilution series of DENV-1 and DENV-3. Two specificity controls consisted of a s