3d quantitative imaging of unprocessed live tissue reveals epithelial defense against bacterial adhesion and subsequent traversal requires myd88未加工的活组织的三维定量成像显示上皮防御细菌粘附和后续遍历需要myd88.pdfVIP

3D Quantitative Imaging of Unprocessed Live Tissue Reveals Epithelial Defense against Bacterial Adhesion and Subsequent Traversal Requires MyD88 1 1 2 3 3 1,4 Connie Tam , Jeffrey LeDue , James J. Mun , Paul Herzmark , Ellen A. Robey , David J. Evans , Suzanne M. J. Fleiszig1,2,5* 1 School of Optometry, University of California, Berkeley, California, United States of America, 2 Program in Vision Science, University of California, Berkeley, California, United States of America, 3 Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America, 4 College of Pharmacy, Touro University California, Vallejo, California, United States of America, 5 Programs in Infectious Diseases and Immunity and Microbiology, University of California, Berkeley, California, United States of America Abstract While a plethora of in vivo models exist for studying infectious disease and its resolution, few enable factors involved in the maintenance of health to be studied in situ. This is due in part to a paucity of tools for studying subtleties of bacterial-host interactions at a cellular level within live organs or tissues, requiring investigators to rely on overt outcomes (e.g. pathology) in their research. Here, a suite of imaging technologies were combined to enable 3D and temporal subcellular localization and quantification of bacterial distribution within the murine cornea without the need for tissue processing or dissection. These methods were then used to demonstrate the importance of MyD88, a central adaptor protein for Toll-Like Receptor (TLR) mediated signaling, in protecting a multilayered epithelium against both adhesion and traversal by the opportunistic bacterial pa