3-dimensional examination of the adult mouse subventricular zone reveals lineage-specific microdomains三维检测的成年老鼠subventricular区显示lineage-specific microdomains.pdfVIP

3-Dimensional Examination of the Adult Mouse Subventricular Zone Reveals Lineage-Specific Microdomains 1 1 1 1 3 Kasum Azim , Roberto Fiorelli , Stefan Zweifel , Anahi Hurtado-Chong , Kazuaki Yoshikawa , 2 1 Lutz Slomianka , Olivier Raineteau * ¨ ¨ ¨ ¨ 1 Brain Research Institute, University of Zurich/ETHZ, Zurich, Switzerland, 2 Institute of Anatomy, University of Zurich, Zurich, Switzerland, 3 Institute for Protein Research Osaka University, Osaka, Japan Abstract Recent studies suggest that the subventricular zone (SVZ) of the lateral ventricle is populated by heterogeneous populations of stem and progenitor cells that, depending on their exact location, are biased to acquire specific neuronal fates. This newly described heterogeneity of SVZ stem and progenitor cells underlines the necessity to develop methods for the accurate quantification of SVZ stem and progenitor subpopulations. In this study, we provide 3-dimensional topographical maps of slow cycling ‘‘stem’’ cells and progenitors based on their unique cell cycle properties. These maps revealed that both cell populations are present throughout the lateral ventricle wall as well as in discrete regions of the dorsal wall. Immunodetection of transcription factors expressed in defined progenitor populations further reveals that divergent lineages have clear regional enrichments in the rostro-caudal as well as in the dorso-ventral span of the lateral ventricle. Thus, progenitors expressing Tbr2 and Dlx2 were confined to dorsal and dorso-lateral r