25(oh)d3 affects the maturation and function of mouse bone marrow-derived dendritic cells stimulated by mycobacterium bovis bcg25(oh)d3影响老鼠骨骨髓来源树突状细胞的成熟和功能刺激牛结核分枝杆菌卡介苗.pdfVIP

25(OH)D3 Affects the Maturation and Function of Mouse Bone Marrow-Derived Dendritic Cells Stimulated by Mycobacterium Bovis BCG 1. 1. 2 1 1 1 Hui-feng Yang , Ze-hua Zhang , Liang-bi Xiang , Kang-lai Tang , Fei Luo , Chun-yu Liu , Jian- 1 1 1 bo Zhou , Jin-qing Li , Jian-zhong Xu * 1 Department of Orthopedic Surgery, Southwest Hospital, Third Military Medical University, Chongqing, People’s Republic of China, 2 Department of Orthopedic Surgery, General Hospital of Shenyang Military Command, Shenyang, People’s Republic of China Abstract It has been shown that vitamin D deficiency increases an individual’s susceptibility to tuberculosis (TB). However, very little is known about the effect of vitamin D on the immune response to Mycobacterium tuberculosis (M. tb) in dendritic cells (DCs). Because DCs play an important role in TB infection, we investigated the phenotypic characteristics and functional ´ capabilities of mouse bone marrow-derived dendritic cells (BMDCs) after stimulation with Bacillus Calmette-Guerin (BCG) in the presence or absence of 25(OH)D3(100 nM). Bone marrow cells from mice were cultured with GM-CSF (20 ng/ml) and were then treated with 25(OH)D3 for 7 days. On day 6, 5 mg/ml of BCG ($1.06106 CFU/mg) was added to the cells for 24 hours, and on day 7, the non-adherent cells were harvested for phenotypic and functional analyses. After incubation with 25(OH)D3, the expression levels of MHC-II and CD86 on the surface of the dendritic cells (DCs) and the ability of the DCs to stimulate proliferation of allogeneic mixed lymphocyt