20-hydroxycholecalciferol, product of vitamin d3 hydroxylation by p450scc, decreases nf-κb activity by increasing iκbα levels in human keratinocytesp450scc 20-hydroxycholecalciferol,维生素d3的产物羟基化,减少nf-κb活动增加iκbα人类角质细胞水平.pdfVIP

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20-hydroxycholecalciferol, product of vitamin d3 hydroxylation by p450scc, decreases nf-κb activity by increasing iκbα levels in human keratinocytesp450scc 20-hydroxycholecalciferol,维生素d3的产物羟基化,减少nf-κb活动增加iκbα人类角质细胞水平.pdf

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20-hydroxycholecalciferol, product of vitamin d3 hydroxylation by p450scc, decreases nf-κb activity by increasing iκbα levels in human keratinocytesp450scc 20-hydroxycholecalciferol,维生素d3的产物羟基化,减少nf-κb活动增加iκbα人类角质细胞水平

20-Hydroxycholecalciferol, Product of Vitamin D3 Hydroxylation by P450scc, Decreases NF-kB Activity by Increasing IkBa Levels in Human Keratinocytes 1 1 2 1 2 Zorica Janjetovic , Michal A. Zmijewski , Robert C. Tuckey , Damon A. DeLeon , Minh N. Nguyen , 1 1 Lawrence M. Pfeffer , Andrzej T. Slominski * 1 Department of Pathology and Laboratory Medicine, the Center for Cancer Research, University of Tennessee Health Science Center, Memphis, Tennessee, United States of America, 2 School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, Crawley, Australia Abstract The side chain of vitamin D3 is hydroxylated in a sequential manner by cytochrome P450scc (CYP11A1) to form 20- hydroxycholecalciferol, which can induce growth arrest and differentiation of both primary and immortalized epidermal keratinocytes. Since nuclear factor-kB (NF-kB) plays a pivotal role in the regulation of cell proliferation, differentiation and apoptosis, we examined the capability of 20-hydroxycholecalciferol to modulate the activity of NF-kB, using 1,25- dihydroxycholecalciferol (calcitriol) as a positive control. 20-hydroxycholecalciferol inhibits the activation of NFkB DNA binding activity as well as NF-kB-driven reporter gene activity in keratinocytes. Also, 20-hydroxycholecalciferol induced significant increases in the mRNA and protein levels of the NF-kB inhibitor protein, IkBa, in a time dependent manner, while no changes in total NF-kB-p65 mRNA or protein levels were observed. Another measure of NF-kB activity, p65 translocation from the cytoplasm into the nucleus was also