a cytochrome b561 with ferric reductase activity from the parasitic blood fluke, schistosoma japonicum寄生的细胞色素与铁还原酶活动b561血吸虫,日本血吸虫.pdfVIP

A Cytochrome b561 with Ferric Reductase Activity from the Parasitic Blood Fluke, Schistosoma japonicum 1,2 1 1 3 4 Amber Glanfield , Donald P. McManus , Danielle J. Smyth , Erica M. Lovas , Alex Loukas , Geoffrey N. Gobert1, Malcolm K. Jones 1,3* 1 Queensland Institute of Medical Research, Herston, Queensland, Australia, 2 School of Population Health, The University of Queensland, Brisbane, Queensland, Australia, 3 School of Veterinary Sciences, The University of Queensland, Brisbane, Queensland, Australia, 4 Queensland Tropical Health Alliance, James Cook University, Cairns, Queensland, Australia Abstract Background: Iron has an integral role in numerous cellular reactions and is required by virtually all organisms. In physiological conditions, iron is abundant in a largely insoluble ferric state. Ferric reductases are an essential component of iron uptake by cells, reducing iron to the soluble ferrous form. Cytochromes b561 (cyts-b561) are a family of ascorbate reducing transmembrane proteins found in most eukaryotic cells. The identification of the ferric reductase duodenal cytochrome b (dcytb) and recent observations that other cyts-b561 may be involved in iron metabolism have opened novel perspectives for elucidating their physiological function. Methodology/Principal Findings: Here we have identified a new member of the cytochrome b561 (Sjcytb561) family in the pathogenic blood fluke Schistosoma japonicum that localises to the outer surface of this parasitic trematode. Heterologous expression of recombinant Sjcyt-b561 in a Saccharomyces cerevisiae mutant strain that lacks plasma membrane ferrireductase activity