a defined, feeder-free, serum-free system to generate in vitro hematopoietic progenitors and differentiated blood cells from hescs and hipscs无血清培养系统定义、feeder-free血液系统产生体外造血祖细胞和分化细胞为hipscs.pdfVIP

A Defined, Feeder-Free, Serum-Free System to Generate In Vitro Hematopoietic Progenitors and Differentiated Blood Cells from hESCs and hiPSCs 1 1 1 1 2 Giorgia Salvagiotto *, Sarah Burton , Christine A. Daigh , Deepika Rajesh , Igor I. Slukvin , Nicholas J. Seay1 1 Department of Research and Development, Cellular Dynamics International, Inc., Madison, Wisconsin, United States of America, 2 Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, Wisconsin, United States of America Abstract Human ESC and iPSC are an attractive source of cells of high quantity and purity to be used to elucidate early human development processes, for drug discovery, and in clinical cell therapy applications. To efficiently differentiate pluripotent cells into a pure population of hematopoietic progenitors we have developed a new 2-dimentional, defined and highly efficient protocol that avoids the use of feeder cells, serum or embryoid body formation. Here we showed that a single matrix protein in combination with growth factors and a hypoxic environment is sufficient to generate from pluripotent cells hematopoietic progenitors capable of differentiating further in mature cell types of different lineages of the blood system. We tested the differentiation method using hESCs and 9 iPSC lines generated from different tissues. These data indicate the robustness of the protocol providing a valuable tool for the generation of clinical-grade hematopoietic cells from pluripotent cells. Citation: Salvagiotto G, Burton S, Daigh CA, Rajesh D, Slukvin II, et al. (2011) A Defined, Feeder-Free, Serum-Free System to Generate In Vitro Hematopoietic Progenitors and Differentiated Blood Cells from hESCs