a fasciclin-like arabinogalactan-protein (fla) mutant of arabidopsis thaliana, fla1, shows defects in shoot regeneration一个fasciclin-like arabinogalactan-protein(佛罗里达州)拟南芥突变体,fla1,显示缺陷射杀再生.pdfVIP

A Fasciclin-Like Arabinogalactan-Protein (FLA) Mutant of Arabidopsis thaliana, fla1, Shows Defects in Shoot Regeneration 1¤ 2,3 1 3 Kim L. Johnson , Natalie A. J. Kibble , Antony Bacic , Carolyn J. Schultz * 1 ARC Centre of Excellence in Plant Cell Walls and Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Melbourne, Australia, 2 ARC Centre of Excellence in Plant Cell Walls, Waite Research Institute, The University of Adelaide, Waite Campus, Glen Osmond, Australia, 3 School of Agriculture and Wine, Waite Research Institute, The University of Adelaide, Waite Campus, Glen Osmond, Australia Abstract Background: The fasciclin-like arabinogalactan-proteins (FLAs) are an enigmatic class of 21 members within the larger family of arabinogalactan-proteins (AGPs) in Arabidopsis thaliana. Located at the cell surface, in the cell wall/plasma membrane, they are implicated in many developmental roles yet their function remains largely undefined. Fasciclin (FAS) domains are putative cell-adhesion domains found in extracellular matrix proteins of organisms from all kingdoms, but the juxtaposition of FAS domains with highly glycosylated AGP domains is unique to plants. Recent studies have started to elucidate the role of FLAs in Arabidopsis development. FLAs containing a single FAS domain are important for the integrity and elasticity of the plant cell wall matrix (FLA11 and FLA12) and FLA3 is involved in microspore development. FLA4/SOS5 with two FAS domains and two AGP domains has a role in maintaining proper cell expansion under salt stressed conditions. The role of other FLAs remains to be uncovered. Method/Principal Findings: Here we describe the characterisat