a gene expression signature of invasive potential in metastatic melanoma cells基因表达签名的侵袭性转移性黑色素瘤细胞的潜能.pdfVIP

A Gene Expression Signature of Invasive Potential in Metastatic Melanoma Cells 1 2 1 3 1 1 Aaron R. Jeffs *, Amy C. Glover , Lynn J. Slobbe , Li Wang , Shujie He , Jody A. Hazlett , Anshul 4 1 5 5 3 5 Awasthi , Adele G. Woolley , Elaine S. Marshall , Wayne R. Joseph , Cristin G. Print , Bruce C. Baguley , Michael R. Eccles1 1 Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand, 2 Department of Biology, University of York, York, United Kingdom, 3 Department of Molecular Medicine and Pathology, School of Medical Sciences, University of Auckland, Auckland, New Zealand, 4 National School of Pharmacy, University of Otago, Dunedin, New Zealand, 5 Auckland Cancer Society Research Centre, University of Auckland, Auckland, New Zealand Abstract Background: We are investigating the molecular basis of melanoma by defining genomic characteristics that correlate with tumour phenotype in a novel panel of metastatic melanoma cell lines. The aim of this study is to identify new prognostic markers and therapeutic targets that might aid clinical cancer diagnosis and management. Principal Findings: Global transcript profiling identified a signature featuring decreased expression of developmental and lineage specification genes including MITF, EDNRB, DCT, and TYR, and increased expression of genes involved in interaction with the extracellular environment, such as PLAUR, VCAN, and HIF1a. Migration assays showed that the gene signature correlated with the invasive potential of the cell lines, and external validation by using publicly available data indicated that