a genome-wide identification analysis of small regulatory rnas in mycobacterium tuberculosis by rna-seq and conservation analysis小的全基因组鉴定分析监管rna在结核分枝杆菌rna-seq和节能分析.pdfVIP

下载本文档

2
0
约 10页
2017-09-04 发布于上海
举报
版权申诉

a genome-wide identification analysis of small regulatory rnas in mycobacterium tuberculosis by rna-seq and conservation analysis小的全基因组鉴定分析监管rna在结核分枝杆菌rna-seq和节能分析.pdf

1、本文档共10页，可阅读全部内容。
2、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

a genome-wide identification analysis of small regulatory rnas in mycobacterium tuberculosis by rna-seq and conservation analysis小的全基因组鉴定分析监管rna在结核分枝杆菌rna-seq和节能分析

A Genome-Wide Identification Analysis of Small Regulatory RNAs in Mycobacterium tuberculosis by RNA- Seq and Conservation Analysis 1. 2. 1 2 1 Danilo Pellin , Paolo Miotto , Alessandro Ambrosi , Daniela Maria Cirillo , Clelia Di Serio * ` 1 University Centre for Statistics in the Biomedical Sciences, Universita Vita-Salute San Raffaele, Milan, Italy, 2 Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy Abstract We propose a new method for smallRNAs (sRNAs) identification. First we build an effective target genome (ETG) by means of a strand-specific procedure. Then we propose a new bioinformatic pipeline based mainly on the combination of two types of information: the first provides an expression map based on RNA-seq data (Reads Map) and the second applies principles of comparative genomics leading to a Conservation Map. By superimposing these two maps, a robust method for the search of sRNAs is obtained. We apply this methodology to investigate sRNAs in Mycobacterium tuberculosis H37Rv. This bioinformatic procedure leads to a total list of 1948 candidate sRNAs. The size of the candidate list is strictly related to the aim of the study and to the technology used during the verification process. We provide performance measures of the algorithm in identifying annotated sRNAs reported in three recent published studies. Citation: Pellin D, Miotto P, Ambrosi A, Cirillo DM, Di Serio C (2012) A Genome-Wide Identification Analysis of Small Regulatory RNAs in Mycobacterium tuberculosis by RNA-Seq and Conservation Analysis. PLoS ONE 7(3): e32723. doi:10.1371/journal.pone.0032723 Editor: Delia Goletti, Natio