a luminal glycoprotein drives dose-dependent diameter expansion of the drosophila melanogaster hindgut tube一个腔的糖蛋白驱动器存在剂量依赖的相关性的黑腹果蝇后肠管直径扩张.pdfVIP

A Luminal Glycoprotein Drives Dose-Dependent Diameter Expansion of the Drosophila melanogaster Hindgut Tube 1 ´ 2. 1. 1. ˚ 1 Zulfeqhar A. Syed , Anne-Laure Bouge , Sunitha Byri , Tina M. Chavoshi , Erika Tang , ´ 2 ¨ 1 1 Herve Bouhin , Iris F. van Dijk-Hard *, Anne Uv * ˆ ´ 1 Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden, 2 Centre des Sciences du Gout et de l’Alimentation, Universite de Bourgogne, Dijon, France Abstract An important step in epithelial organ development is size maturation of the organ lumen to attain correct dimensions. Here we show that the regulated expression of Tenectin (Tnc) is critical to shape the Drosophila melanogaster hindgut tube. Tnc is a secreted protein that fills the embryonic hindgut lumen during tube diameter expansion. Inside the lumen, Tnc contributes to detectable O-Glycans and forms a dense striated matrix. Loss of tnc causes a narrow hindgut tube, while Tnc over-expression drives tube dilation in a dose-dependent manner. Cellular analyses show that luminal accumulation of Tnc causes an increase in inner and outer tube diameter, and cell flattening within the tube wall, similar to the effects of a hydrostatic pressure in other systems. When Tnc expression is induced only in cells at one side of the tube wall, Tnc fills the lumen and equally affects all cells at the lumen perimeter, arguing that Tnc acts non-cell-autonomously. Moreover