a mathematical model of mitotic exit in budding yeast the role of polo kinase一个数学模型的有丝分裂退出出芽酵母马球激酶的作用.pdfVIP

A Mathematical Model of Mitotic Exit in Budding Yeast: The Role of Polo Kinase ¤ Baris Hancioglu* , John J. Tyson Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America Abstract Cell cycle progression in eukaryotes is regulated by periodic activation and inactivation of a family of cyclin–dependent kinases (Cdk’s). Entry into mitosis requires phosphorylation of many proteins targeted by mitotic Cdk, and exit from mitosis requires proteolysis of mitotic cyclins and dephosphorylation of their targeted proteins. Mitotic exit in budding yeast is known to involve the interplay of mitotic kinases (Cdk and Polo kinases) and phosphatases (Cdc55/PP2A and Cdc14), as well as the action of the anaphase promoting complex (APC) in degrading specific proteins in anaphase and telophase. To understand the intricacies of this mechanism, we propose a mathematical model for the molecular events during mitotic exit in budding yeast. The model captures the dynamics of this network in wild-type yeast cells and 110 mutant strains. The model clarifies the roles of Polo-like kinase (Cdc5) in the Cdc14 early anaphase release pathway and in the G-protein regulated mitotic exit network. Citation: Hancioglu B, Tyson JJ (2012) A Mathematical Model of Mitotic Exit in Budding Yeast: The Role of Polo Kinase. PLoS ONE 7(2): e30810. doi:10.1371/ journal.pone.0030810 Editor: Michael Lichten, National Cancer Institute, United States of America Received October 8, 2011; Accepted December 21, 2011; Published February 23, 2012 Copyright: 2012 Hancioglu, Tyson. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the ori