a method for the direct identification of differentiating muscle cells by a fluorescent mitochondrial dye直接识别的方法区分肌肉细胞线粒体荧光染料.pdfVIP

A Method for the Direct Identification of Differentiating Muscle Cells by a Fluorescent Mitochondrial Dye 1 2 1 Tetsuaki Miyake *, John C. McDermott , Anthony O. Gramolini * 1 Department of Physiology, University of Toronto, Best Institute Medical Research, Toronto, Canada, 2 Department of Biology, York University, Toronto, Canada Abstract Identification of differentiating muscle cells generally requires fixation, antibodies directed against muscle specific proteins, and lengthy staining processes or, alternatively, transfection of muscle specific reporter genes driving GFP expression. In this study, we examined the possibility of using the robust mitochondrial network seen in maturing muscle cells as a marker of cellular differentiation. The mitochondrial fluorescent tracking dye, MitoTracker, which is a cell-permeable, low toxicity, fluorescent dye, allowed us to distinguish and track living differentiating muscle cells visually by epi-fluorescence microscopy. MitoTracker staining provides a robust and simple detection strategy for living differentiating cells in culture without the need for fixation or biochemical processing. Citation: Miyake T, McDermott JC, Gramolini AO (2011) A Method for the Direct Identification of Differentiating Muscle Cells by a Fluorescent Mitochondrial Dye. PLoS ONE 6(12): e28628. doi:10.1371/journal.pone.0028628 Editor: Antonio Paolo Beltrami, University of Udine, Italy Received August 2, 2011; Accepted November 11, 2011; Published December 9, 2011 Copyright: 2011 Miyake et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and sou