a molecular assay for sensitive detection of pathogen-specific t-cells分子检测为敏感pathogen-specific t细胞的检测.pdfVIP
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a molecular assay for sensitive detection of pathogen-specific t-cells分子检测为敏感pathogen-specific t细胞的检测
A Molecular Assay for Sensitive Detection of Pathogen-
Specific T-Cells
Victoria O. Kasprowicz1,2,3*, Jessica E. Mitchell1,2,3, Shivan Chetty2,3, Pamla Govender2,3, Kuan-Hsiang
5 4 5 5 6
Gary Huang , Helen A. Fletcher , Daniel P. Webster , Sebastian Brown , Anne Kasmar , Kerry
7 3 3 1,2,3 3
Millington , Cheryl L. Day , Nompumelelo Mkhwanazi , Cheryl McClurg , Fundisiwe Chonco , Ajit
Lalvani7, Bruce D. Walker1,3, Thumbi Ndung’u1,3, Paul Klenerman5
1 Ragon Institute of MGH, MIT and Harvard, Harvard Medical School, Boston, Massachusetts, United States of America, 2 Kwazulu-Natal Research Institute for TB and HIV,
Nelson R. Mandela School of Medicine, Durban, South Africa, 3 HIV Pathogenesis Programme, Doris Duke Medical Research Institute, Nelson R. Mandela School of
Medicine, Durban, South Africa, 4 Nuffield Department of Medicine, The Jenner Institute, University of Oxford, Oxford, United Kingdom, 5 Nuffield Department of
Medicine, Oxford Biomedical Research Centre and James Martin School for 21st Century, University of Oxford, Oxford, United Kingdom, 6 Division of Rheumatology,
Immunology and Allergy, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, United States of America, 7 Tuberculosis Research Unit,
Department of Respiratory Medicine, National Heart and Lung Institute, Imperial College London, London, United Kingdom
Abstract
Here we describe the development and validation of a highly sensitive assay of antigen-specific IFN-c production using real
time quantitative PCR (qPCR) for two reporters - monokine-
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