a molecular assay for sensitive detection of pathogen-specific t-cells分子检测为敏感pathogen-specific t细胞的检测.pdfVIP

a molecular assay for sensitive detection of pathogen-specific t-cells分子检测为敏感pathogen-specific t细胞的检测.pdf

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a molecular assay for sensitive detection of pathogen-specific t-cells分子检测为敏感pathogen-specific t细胞的检测

A Molecular Assay for Sensitive Detection of Pathogen- Specific T-Cells Victoria O. Kasprowicz1,2,3*, Jessica E. Mitchell1,2,3, Shivan Chetty2,3, Pamla Govender2,3, Kuan-Hsiang 5 4 5 5 6 Gary Huang , Helen A. Fletcher , Daniel P. Webster , Sebastian Brown , Anne Kasmar , Kerry 7 3 3 1,2,3 3 Millington , Cheryl L. Day , Nompumelelo Mkhwanazi , Cheryl McClurg , Fundisiwe Chonco , Ajit Lalvani7, Bruce D. Walker1,3, Thumbi Ndung’u1,3, Paul Klenerman5 1 Ragon Institute of MGH, MIT and Harvard, Harvard Medical School, Boston, Massachusetts, United States of America, 2 Kwazulu-Natal Research Institute for TB and HIV, Nelson R. Mandela School of Medicine, Durban, South Africa, 3 HIV Pathogenesis Programme, Doris Duke Medical Research Institute, Nelson R. Mandela School of Medicine, Durban, South Africa, 4 Nuffield Department of Medicine, The Jenner Institute, University of Oxford, Oxford, United Kingdom, 5 Nuffield Department of Medicine, Oxford Biomedical Research Centre and James Martin School for 21st Century, University of Oxford, Oxford, United Kingdom, 6 Division of Rheumatology, Immunology and Allergy, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, United States of America, 7 Tuberculosis Research Unit, Department of Respiratory Medicine, National Heart and Lung Institute, Imperial College London, London, United Kingdom Abstract Here we describe the development and validation of a highly sensitive assay of antigen-specific IFN-c production using real time quantitative PCR (qPCR) for two reporters - monokine-

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