a multi-population consensus genetic map reveals inconsistent marker order among maps likely attributed to structural variations in the apple genomemulti-population共识遗传图谱显示不一致的地图标记次序可能归因于苹果基因组结构的变化.pdfVIP

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2017-09-05 发布于上海
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a multi-population consensus genetic map reveals inconsistent marker order among maps likely attributed to structural variations in the apple genomemulti-population共识遗传图谱显示不一致的地图标记次序可能归因于苹果基因组结构的变化.pdf

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a multi-population consensus genetic map reveals inconsistent marker order among maps likely attributed to structural variations in the apple genomemulti-population共识遗传图谱显示不一致的地图标记次序可能归因于苹果基因组结构的变化

A Multi-Population Consensus Genetic Map Reveals Inconsistent Marker Order among Maps Likely Attributed to Structural Variations in the Apple Genome 1 2 3 4 1 Muhammad Awais Khan , Yuepeng Han , Youfu Frank Zhao , Michela Troggio , Schuyler S. Korban * 1 Department of Natural Resources Environmental Sciences, University of Illinois, Urbana, Illinois, United States of America, 2 Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Moshan, Wuhan, People’s Republic of China, 3 Department of Crop Sciences, University of Illinois, Urbana, Illinois, United States of America, 4 Istituto Agrario San Michele all’Adige Research and Innovation Centre, Foundation Edmund Mach, Trento, Italy Abstract Genetic maps serve as frameworks for determining the genetic architecture of quantitative traits, assessing structure of a genome, as well as aid in pursuing association mapping and comparative genetic studies. In this study, a dense genetic map was constructed using a high-throughput 1,536 EST-derived SNP GoldenGate genotyping platform and a global consensus map established by combining the new genetic map with four existing reliable genetic maps of apple. The consensus map identified markers with both major and minor conflicts in positioning across all five maps. These major inconsistencies among marker positions were attributed either to structural variations within the apple genome, or among mapping populations, or genotyping technical errors. These also highlighted problems in assembly and anchorage of the reference draft apple genome sequence in regions with known segmental duplications. Markers common across all five apple gen