a multi-step pathway for the establishment of sister chromatid cohesion一个多步骤的途径建立姊妹染色单体凝聚力.pdfVIP
- 1、本文档共12页,可阅读全部内容。
- 2、有哪些信誉好的足球投注网站(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
a multi-step pathway for the establishment of sister chromatid cohesion一个多步骤的途径建立姊妹染色单体凝聚力
A Multi-Step Pathway for the Establishment of
Sister Chromatid Cohesion
1,2,3 ¨ 1,2,3 1,2,4 5 1,2,3*
Mark Milutinovich , Elc¸in Unal , Chris Ward , Robert V. Skibbens , Douglas Koshland
1 Howard Hughes Medical Institute, Carnegie Institution, Baltimore, Maryland, United States of America, 2 Department of Embryology, Carnegie Institution, Baltimore,
Maryland, United States of America, 3 Department of Biology, Johns Hopkins University, Baltimore, Maryland, United States of America, 4 Ingenuity Program, Baltimore
Polytechnic Institute, Baltimore, Maryland, United States of America, 5 Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania, United States of
America
The cohesion of sister chromatids is mediated by cohesin, a protein complex containing members of the structural
maintenance of chromosome (Smc) family. How cohesins tether sister chromatids is not yet understood. Here, we
mutate SMC1, the gene encoding a cohesin subunit of budding yeast, by random insertion dominant negative
mutagenesis to generate alleles that are highly informative for cohesin assembly and function. Cohesins mutated in
the Hinge or Loop1 regions of Smc1 bind chromatin by a mechanism similar to wild-type cohesin, but fail to enrich at
cohesin-associated regions (CARs) and pericentric regions. Hence, the Hinge and Loop1 regions of Smc1 are essential
for the specific chromatin binding of cohesin. This specific binding and a subsequent Ctf7/Eco1-dependent step are
both required for the establishment of cohesion. We propose that a cohesin or cohesin oligomer tethers the sister
chromatids through two chromatin-binding events that are regulated spatially by CAR binding and temporally by Ctf7
activat
您可能关注的文档
- a dominant mutation in mediator of paramutation2, one of three second-largest subunits of a plant-specific rna polymerase, disrupts multiple sirna silencing processes中介的paramutation2显性突变,三种植物的rna聚合酶的第二大亚基,扰乱了小干扰rna沉默多个流程.pdf
- a dominant negative zebrafish ahr2 partially protects developing zebrafish from dioxin toxicity占主导地位的消极斑马鱼ahr2部分保护发展中斑马鱼二恶英的毒性.pdf
- a double-blind, placebo-controlled, randomized, clinical trial of the tlr-3 agonist rintatolimod in severe cases of chronic fatigue syndrome一项双盲、安慰剂对照、随机临床试验的tlr-3兴奋剂rintatolimod慢性疲劳综合症的严重病例.pdf
- a dominantly acting murine allele of mcm4 causes chromosomal abnormalities and promotes tumorigenesis主代理小鼠等位基因mcm4导致染色体异常和促进肿瘤发生.pdf
- a dopa decarboxylase modulating the immune response of scallop chlamys farreri多巴脱羧酶调节免疫应答的扇贝斗篷farreri.pdf
- a drastic reduction in the life span of cystatin c l68q carriers due to life-style changes during the last two centuries的寿命大大降低半胱氨酸蛋白酶抑制物c l68q运营商由于生活方式的改变在过去的两个世纪.pdf
- a double-edged sword role for ubiquitin-proteasome system in brain stem cardiovascular regulation during experimental brain death一把双刃剑作用ubiquitin-proteasome系统在脑干心血管调节实验性脑死亡.pdf
- a downstream cpg island controls transcript initiation and elongation and the methylation state of the imprinted airn macro ncrna promoter下游cpg岛控制转录起始和延伸和印airn宏观ncrna启动子的甲基化状态.pdf
- a drosophila pattern recognition receptor contains a peptidoglycan docking groove and unusual l,d-carboxypeptidase activity果蝇模式识别受体包含一个肽聚糖对接坡口和不寻常的l,d-carboxypeptidase活动.pdf
- a drosophila smyd4 homologue is a muscle-specific transcriptional modulator involved in development果蝇smyd4同系物是阳性转录调制器参与发展.pdf
文档评论(0)