a multi-cell, multi-scale model of vertebrate segmentation and somite formation脊椎动物的多单元、多尺度模型分割和体节形成.pdfVIP

A Multi-cell, Multi-scale Model of Vertebrate Segmentation and Somite Formation Susan D. Hester*, Julio M. Belmonte, J. Scott Gens, Sherry G. Clendenon, James A. Glazier Biocomplexity Institute and Department of Physics, Indiana University Bloomington, Bloomington, Indiana, United States of America Abstract Somitogenesis, the formation of the body’s primary segmental structure common to all vertebrate development, requires coordination between biological mechanisms at several scales. Explaining how these mechanisms interact across scales and how events are coordinated in space and time is necessary for a complete understanding of somitogenesis and its evolutionary flexibility. So far, mechanisms of somitogenesis have been studied independently. To test the consistency, integrability and combined explanatory power of current prevailing hypotheses, we built an integrated clock-and-wavefront model including submodels of the intracellular segmentation clock, intercellular segmentation-clock coupling via Delta/ Notch signaling, an FGF8 determination front, delayed differentiation, clock-wavefront readout, and differential-cell-cell- adhesion-driven cell sorting. We identify inconsistencies between existing submodels and gaps in the current understanding of somitogenesis mechanisms, and propose novel submodels and extensions of existing submodels where necessary. For reasonable initial conditions, 2D simulations of our model robustly generate spatially and temporally regular somites, realistic dynamic morphologies and spontaneous emergence of anterior-traveling stripes of Lfng. We show that these traveling stripes are pseudo-waves rather than true propagating waves. Our model is flexible enough to generate interspecies-like variation in somite size in response to changes in the PSM growth