a new approach to dengue fatal cases diagnosis ns1 antigen capture in tissues新方法登革热死亡病例诊断ns1抗原捕获在组织.pdfVIP

A New Approach to Dengue Fatal Cases Diagnosis: NS1 Antigen Capture in Tissues 1 1 1 Monique da Rocha Queiroz Lima , Rita Maria Ribeiro Nogueira , Hermann Gonc¸alves Schatzmayr , Ana 1 2 1 Maria Bispo de Filippis , Daniel Limonta , Flavia Barreto dos Santos * 1 Flavivirus Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil, 2 National Reference Laboratory for Dengue, Virology Department, PAHO/WHO Collaborating Center ´ for the Study of Dengue and Its Vector, Pedro Kourı Institute of Tropical Medicine, Havana, Cuba Abstract Abstract/Background: Dengue is the most important arthropod borne viral disease worldwide in terms of morbidity and mortality and is caused by any of the four serotypes of dengue virus (DENV-1 to 4). Brazil is responsible for approximately 80% of dengue cases in the Americas, and since the introduction of dengue in 1986, a total of 5,944,270 cases have been reported including 21,596 dengue hemorrhagic fever and 874 fatal cases. DENV can infect many cell types and cause diverse clinical and pathological effects. The goal of the study was to investigate the usefulness of NS1 capture tests as an alternative tool to detect DENV in tissue specimens from previously confirmed dengue fatal cases (n = 23) that occurred in 2002 in Brazil. Methodology/Principal Findings: A total of 74 tissue specimens were available: liver (n = 23), lung (n = 14), kidney (n = 04), brain (n = 10), heart (n = 02), skin (n = 01), spleen (n = 15), thymus (n = 03) and lymph nodes (n = 02). We evaluated three tests for NS1 antigen capture: first generation Dengue Early