a new pcr-based approach indicates the range of clonorchis sinensis now extends to central thailand一个新的pcr方法表明clonorchis sinensis现在的范围延伸到中央泰国.pdfVIP

A New PCR-Based Approach Indicates the Range of Clonorchis sinensis Now Extends to Central Thailand 1 1 2 2 3 Rebecca J. Traub *, Julie Macaranas , Mathirut Mungthin , Saovanee Leelayoova , Thomas Cribb , K. Darwin Murrell4, R. C. Andrew Thompson5 1 School of Veterinary Science, University of Queensland, St. Lucia, Queensland, Australia, 2 Department of Parasitology, Phramongkutklao College of Medicine, Bangkok, Thailand, 3 School of Molecular and Microbial Sciences and Centre for Marine Studies, University of Queensland, St Lucia, Queensland, Australia, 4 Department of Veterinary Pathobiology, Faculty of Life Sciences, University of Copenhagen, Fredericksberg, Denmark, 5 World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia, Australia Abstract Differentiation of the fish-borne trematodes belonging to the Opisthorchiidae, Heterophyidae and Lecithodendriidae is important from a clinical and epidemiological perspective, yet it is impossible to do using conventional coprological techniques, as the eggs are morphologically similar. Epidemiological investigation therefore currently relies on morphological examination of adult worms following expulsion chemotherapy. A PCR test capable of amplifying a segment of the internal transcribed spacer region of ribosomal DNA for the opisthorchiid and heterophyid flukes eggs taken directly from faeces was developed and evaluated in a rural community in central Thailand. The lowest quantity of DNA that could be amplified from individual adults of Opisth