a new role for an old antimicrobial lysozyme c-1 can function to protect malaria parasites in anopheles mosquitoes新角色老颈- 1抗菌溶菌酶可以在按蚊功能保护疟疾寄生虫.pdfVIP

A New Role for an Old Antimicrobial: Lysozyme c-1 Can Function to Protect Malaria Parasites in Anopheles Mosquitoes 1. 1,2. 1,3 4 5 1 Mayur K. Kajla , Lei Shi , Bin Li , Shirley Luckhart , Jianyong Li , Susan M. Paskewitz * 1 Department of Entomology, University of Wisconsin, Madison, Wisconsin, United States of America, 2 Section of Allergy, Pulmonary and Critical Care, Department of Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States of America, 3 Section of Microbiology, College of Biological Sciences, University of California Davis, Davis, California, United States of America, 4 Department of Medical Microbiology and Immunology, University of California Davis, School of Medicine, Davis, California, United States of America, 5 Department of Biochemistry, Virginia Tech, Blacksburg, Virginia, United States of America Abstract Background: Plasmodium requires an obligatory life stage in its mosquito host. The parasites encounter a number of insults while journeying through this host and have developed mechanisms to avoid host defenses. Lysozymes are a family of important antimicrobial immune effectors produced by mosquitoes in response to microbial challenge. Methodology/Principal Findings: A mosquito lysozyme was identified as a protective agonist for Plasmodium. Immunohistochemical analyses demonstrated that Anopheles gambiae lysozyme c-1 binds to oocysts of Plasmodium berghei and Plasmodium falciparum at 2 and 5 days after infection. Similar results were observed with Anopheles stephensi and P. falciparum, suggesting wide occurrence of this phenomenon across parasite and vector species. Lysozyme c-1 did not