a new strategy to generate functional insulin-producing cell lines by somatic gene transfer into pancreatic progenitors一个新的策略来生成功能产生胰岛素细胞系通过体细胞基因转移到胰腺祖细胞.pdfVIP

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a new strategy to generate functional insulin-producing cell lines by somatic gene transfer into pancreatic progenitors一个新的策略来生成功能产生胰岛素细胞系通过体细胞基因转移到胰腺祖细胞.pdf

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a new strategy to generate functional insulin-producing cell lines by somatic gene transfer into pancreatic progenitors一个新的策略来生成功能产生胰岛素细胞系通过体细胞基因转移到胰腺祖细胞

A New Strategy to Generate Functional Insulin- Producing Cell Lines by Somatic Gene Transfer into Pancreatic Progenitors 1 1,2 1,2 2,3 Philippe Ravassard *, Emilie Bricout-Neveu , Yasmine Hazhouz , Severine Pechberty , Jacques 1 2 3 Mallet , Paul Czernichow , Raphael Scharfmann * ´ 1 Biotechnology Biotherapy group Centre de Recherche Institut du Cerveau et de la Moelle CNRS UMR7225, INSERM UMRS795, Universite Pierre et Marie Curie, Paris, ´ ´ ´ ˆ France, 2 Endocells, Paris, France, 3 INSERM U845, Centre de Recherche Croissance et Signalisation, Universite Paris Descartes, Faculte de Medecine, Hopital Necker, Paris, France Abstract Background: There is increasing interest in developing human cell lines to be used to better understand cell biology, but also for drug screening, toxicology analysis and future cell therapy. In the endocrine pancreatic field, functional human beta cell lines are extremely scarce. On the other hand, rodent insulin producing beta cells have been generated during the past years with great success. Many of such cell lines were produced by using transgenic mice expressing SV40T antigen under the control of the insulin promoter, an approach clearly inadequate in human. Our objective was to develop and validate in rodent an alternative transgenic-like approach, applicable to human tissue, by performing somatic gene transfer into pancre