a novel two-component system involved in secretion stress response in streptomyces lividans一种新型双组分系统参与分泌压力反应链霉菌属lividans.pdfVIP

A Novel Two-Component System Involved in Secretion Stress Response in Streptomyces lividans ´ Sonia Gullon, Rebeca L. Vicente, Rafael P. Mellado* ´ Centro Nacional de Biotecnologıa (CNB-CSIC), Madrid, Spain Abstract Background: Misfolded proteins accumulating outside the bacterial cytoplasmic membrane can interfere with the secretory machinery, hence the existence of quality factors to eliminate these misfolded proteins is of capital importance in bacteria that are efficient producers of secretory proteins. These bacteria normally use a specific two-component system to respond to the stress produced by the accumulation of the misfolded proteins, by activating the expression of HtrA-like proteases to specifically eliminate the incorrectly folded proteins. Methodology/Principal Findings: Overproduction of alpha-amylase in S. lividans causing secretion stress permitted the identification of a two-component system (SCO4156-SCO4155) that regulates three HtrA-like proteases which appear to be involved in secretion stress response. Mutants in each of the genes forming part of the two-genes operon that encodes the sensor and regulator protein components accumulated misfolded proteins outside the cell, strongly suggesting the involvement of this two-component system in the S. lividans secretion stress response. Conclusions/Significance: To our knowledge this is the first time that a specific secretion stress response two-component system is found to control the expression of three HtrA-like protease genes in S. lividans, a bacterium that has been repeatedly used as a host for the synthesis of homologous and heterologous secretory proteins of industrial application. ´ Citation: Gullon S, Vicente RL, Mellado RP (2012) A Novel Tw