a proteomic approach for the diagnosis of bacterial meningitis蛋白质组学的方法对细菌性脑膜炎的诊断.pdfVIP

A Proteomic Approach for the Diagnosis of Bacterial Meningitis 1 1 1 1 1 1 Sarah Jesse , Petra Steinacker , Stefan Lehnert , Martin Sdzuj , Lukas Cepek , Hayrettin Tumani , Olaf 3,4 2 1 Jahn , Holger Schmidt , Markus Otto * 1 Department of Neurology, University of Ulm, Ulm, Germany, 2 Department of Neurology, University of Goettingen, Goettingen, Germany, 3 DFG Research Center for Molecular Physiology of the Brain, Goettingen, Germany, 4 Proteomics Group, Max-Planck-Institute for Experimental Medicine, Goettingen, Germany Abstract Background: The discrimination of bacterial meningitis (BM) versus viral meningitis (VM) shapes up as a problem, when laboratory data are not equivocal, in particular, when Gram stain is negative. Methodology/Principal Findings: With the aim to determine reliable marker for bacterial or viral meningitis, we subjected cerebrospinal fluid (CSF) to a quantitative proteomic screening. By using a recently established 2D-DIGE protocol which was adapted to the individual CSF flow, we compared a small set of patients with proven BM and VM. Thereby, we identified six potential biomarkers out of which Prostaglandin-H2 D-isomerase was already described in BM, showing proof of concept. In the subsequent validation phase on a more comprehensive collective of 80 patients, we could validate that in BM high levels of glial fibrillary acidic protein (GFAP) and low levels of soluble amyloid precursor protein alpha/beta (sAPPa/ b) are present as possible binding partner of Fibulin-1. Conclusions/Significance: We conclude that our CSF flow-adapted 2D-DIGE protocol is valid especially in comparing samples with high differences in total protein a