a proteomic and cellular analysis of uropods in the pathogen entamoeba histolytica蛋白质组学和细胞分析病原体的腹足痢疾阿米巴.pdfVIP

A Proteomic and Cellular Analysis of Uropods in the Pathogen Entamoeba histolytica Jacques Marquay Markiewicz1,2, Sylvie Syan1,2, Chung-Chau Hon1,2, Christian Weber1,2, Daniela Faust1,2, Nancy Guillen1,2* ´ 1 Institut Pasteur, Unite Biologie Cellulaire du Parasitisme, Paris, France, 2 INSERM U786, Paris, France Abstract Exposure of Entamoeba histolytica to specific ligands induces cell polarization via the activation of signalling pathways and cytoskeletal elements. The process leads to formation of a protruding pseudopod at the front of the cell and a retracting uropod at the rear. In the present study, we show that the uropod forms during the exposure of trophozoites to serum isolated from humans suffering of amoebiasis. To investigate uropod assembly, we used LC-MS/MS technology to identify protein components in isolated uropod fractions. The galactose/N-acetylgalactosamine lectin, the immunodominant antigen M17 (which is specifically recognized by serum from amoeba-infected persons) and a few other cells adhesion- related molecules were primarily involved. Actin-rich cytoskeleton components, GTPases from the Rac and Rab families, filamin, a-actinin and a newly identified ezrin-moesin-radixin protein were the main factors found to potentially interact with capped receptors. A set of specific cysteine proteases and a serine protease were enriched in isolated uropod fractions. However, biological assays indicated that cysteine proteases are not involved in uropod formation in E. histolytica, a fact in contrast to the situation in human motile immune cells. The surface proteins identified here are testable biomarkers which may be either recognized by the immune system and/or released into the circulation during amoebiasis. Citation: Marquay Markiewicz J, Syan S, Hon C-C, Weber C, Faust D,