a quantitative method to analyze drosophila pupal eye patterning定量方法分析果蝇蛹的模式.pdfVIP

A Quantitative Method to Analyze Drosophila Pupal Eye Patterning Ruth I. Johnson, Ross L. Cagan* Department of Developmental and Regenerative Biology, Mount Sinai Medical School, New York, New York, United States of America Abstract Background: The Drosophila pupal eye has become a popular paradigm for understanding morphogenesis and tissue patterning. Correct rearrangement of cells between ommatidia is required to organize the ommatidial array across the eye field. This requires cell movement, cell death, changes to cell-cell adhesion, signaling and fate specification. Methodology: We describe a method to quantitatively assess mis-patterning of the Drosophila pupal eye and objectively calculate a ‘mis-patterning score’ characteristic of a specific genotype. This entails step-by-step scoring of specific traits observed in pupal eyes dissected 40–42 hours after puparium formation and subsequent statistical analysis of this data. Significance: This method provides an unbiased quantitative score of mis-patterning severity that can be used to compare the impact of different genetic mutations on tissue patterning. Citation: Johnson RI, Cagan RL (2009) A Quantitative Method to Analyze Drosophila Pupal Eye Patterning. PLoS ONE 4(9): e7008. doi:10.1371/journal.pone.0007008 Editor: Thomas A. Reh, University of Washington, United States of America Received June 29, 2009; Accepted August 17, 2009; Published September 15, 2009 Copyright: 2009 Johnson, Cagan. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by grant NIH R01 EY1149. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of