a tale of two tissues atgh9c1 is an endo-β-1,4-glucanase involved in root hair and endosperm development in arabidopsis两个组织的故事atgh9c1 endo-β-1,4-glucanase参与拟南芥根头发和胚乳发展.pdfVIP

a tale of two tissues atgh9c1 is an endo-β-1,4-glucanase involved in root hair and endosperm development in arabidopsis两个组织的故事atgh9c1 endo-β-1,4-glucanase参与拟南芥根头发和胚乳发展.pdf

  1. 1、本文档共16页,可阅读全部内容。
  2. 2、有哪些信誉好的足球投注网站(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
  3. 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载
  4. 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
  5. 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
  6. 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们
  7. 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
  8. 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
a tale of two tissues atgh9c1 is an endo-β-1,4-glucanase involved in root hair and endosperm development in arabidopsis两个组织的故事atgh9c1 endo-β-1,4-glucanase参与拟南芥根头发和胚乳发展

A Tale of Two Tissues: AtGH9C1 Is an Endo-b-1,4- Glucanase Involved in Root Hair and Endosperm Development in Arabidopsis Elena del Campillo*, Sivacharan Gaddam, Dorinne Mettle-Amuah, Jean Heneks Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, United States of America Abstract Arabidopsis AtGH9C1 is an endo-b-1,4-glucanase possessing a carbohydrate-binding domain (CBM49). Analysis of AtGH9C1 expression by promoter-reporter GUS, RT-PCR, public transcriptome databases and GFP protein tagging demonstrated a high and selective expression of AtGH9C1 in root hairs and in the endosperm. Expression in root hair cells started prior to bulge formation and continued during hair elongation. AtGH9C1 expression increased with treatments that increase density (ACC) or length (sucrose) of root hairs. Expression in the endosperm extended sequentially to the micropylar, peripheral and chalazal compartments. A mutant with reduced AtGH9C1 expression had a delay in germination and a marked reduction in root hair presence. Complementation of the mutant partially improved both germination and root hair density. Experiments with ectopically expressed AtGH9C1-GFP with and without the CBM49, demonstrated that both forms of the protein are secreted and that CBM49 targets the protein to specific regions of the cell wall, but what makes these regions special is still unknown. The amino acid alignment of angiosperm GH9 genes with C-terminal extensions illustrate that AtGH9C1 belongs to a different clade than its tomato homolog, S1GH9C1. The latter has a CBM49 that was shown to bind crystalline cellulose. We suggest that AtGH9C1 is associated with the weakening of the cell wall during formation and growth of the root hair as well as with the sequential anterior-posterior breakdown of the endosperm cell wall that provides space for the growing

您可能关注的文档

文档评论(0)

qianqiana + 关注
实名认证
文档贡献者

该用户很懒,什么也没介绍

版权声明书
用户编号:5132241303000003

1亿VIP精品文档

相关文档