a tale of two tissues atgh9c1 is an endo-β-1,4-glucanase involved in root hair and endosperm development in arabidopsis两个组织的故事atgh9c1 endo-β-1,4-glucanase参与拟南芥根头发和胚乳发展.pdfVIP
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a tale of two tissues atgh9c1 is an endo-β-1,4-glucanase involved in root hair and endosperm development in arabidopsis两个组织的故事atgh9c1 endo-β-1,4-glucanase参与拟南芥根头发和胚乳发展
A Tale of Two Tissues: AtGH9C1 Is an Endo-b-1,4-
Glucanase Involved in Root Hair and Endosperm
Development in Arabidopsis
Elena del Campillo*, Sivacharan Gaddam, Dorinne Mettle-Amuah, Jean Heneks
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, United States of America
Abstract
Arabidopsis AtGH9C1 is an endo-b-1,4-glucanase possessing a carbohydrate-binding domain (CBM49). Analysis of AtGH9C1
expression by promoter-reporter GUS, RT-PCR, public transcriptome databases and GFP protein tagging demonstrated
a high and selective expression of AtGH9C1 in root hairs and in the endosperm. Expression in root hair cells started prior to
bulge formation and continued during hair elongation. AtGH9C1 expression increased with treatments that increase density
(ACC) or length (sucrose) of root hairs. Expression in the endosperm extended sequentially to the micropylar, peripheral and
chalazal compartments. A mutant with reduced AtGH9C1 expression had a delay in germination and a marked reduction in
root hair presence. Complementation of the mutant partially improved both germination and root hair density. Experiments
with ectopically expressed AtGH9C1-GFP with and without the CBM49, demonstrated that both forms of the protein are
secreted and that CBM49 targets the protein to specific regions of the cell wall, but what makes these regions special is still
unknown. The amino acid alignment of angiosperm GH9 genes with C-terminal extensions illustrate that AtGH9C1 belongs
to a different clade than its tomato homolog, S1GH9C1. The latter has a CBM49 that was shown to bind crystalline cellulose.
We suggest that AtGH9C1 is associated with the weakening of the cell wall during formation and growth of the root hair as
well as with the sequential anterior-posterior breakdown of the endosperm cell wall that provides space for the growing
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