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a transcriptional enhancer from the coding region of adamts5一个转录增强器adamts5的编码区
A Transcriptional Enhancer from the Coding Region of
ADAMTS5
Kristen K. B. Barthel, Xuedong Liu*
Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado, United States of America
Abstract
Background: The revelation that the human genome encodes only ,25,000 genes and thus cannot account for phenotypic
complexity has been one of the biggest surprises in the post-genomic era. However, accumulating evidence suggests that
transcriptional regulation may be in large part responsible for this observed mammalian complexity. Consequently, there
has been a strong drive to locate cis-regulatory regions in mammalian genomes in order to understand the unifying
principles governing these regions, including their genomic distribution. Although a number of systematic approaches have
been developed, these all discount coding sequence.
Methodology/Principal Findings: Using the computational tool PRI (Pattern-defined Regulatory Islands), which does not
mask coding sequence, we identified a regulatory region associated with the gene ADAMTS5 that encompasses the entirety
of the essential coding exon 2. We demonstrate through a combination of chromatin immunoprecipitation and reporter
gene studies that this region can not only bind the myogenic transcription factors MYOD and myogenin and the E-protein
HEB but can also function as a very strong myogenic transcriptional enhancer.
Conclusions/Significance: Thus, we report the identification and detailed characterization of an exonic enhancer.
Ultimately, this leads to the interesting question of why evolution would be so parsimonious in the functional assignment of
sequence.
Citation: Barthel KKB, Liu X (2008) A Transcriptional Enhancer from the Coding Region of ADAMTS5 . PLoS ONE 3(5): e2184. doi:10.1371/journal.pone.0002184
Editor: Edathara Abraham,
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