a three-dimensional comparison of tick-borne flavivirus infection in mammalian and tick cell lines一个三维的比较蜱传播的黄病毒感染哺乳动物和蜱虫细胞系.pdfVIP
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a three-dimensional comparison of tick-borne flavivirus infection in mammalian and tick cell lines一个三维的比较蜱传播的黄病毒感染哺乳动物和蜱虫细胞系
A Three-Dimensional Comparison of Tick-Borne
Flavivirus Infection in Mammalian and Tick Cell Lines
1 2 2 1
Danielle K. Offerdahl , David W. Dorward , Bryan T. Hansen , Marshall E. Bloom *
1 Laboratory of Virology, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana, United States of America, 2 Microscopy Unit, Research Technology Branch, Rocky
Mountain Laboratories, NIAID, NIH, Hamilton, Montana, United States of America
Abstract
Tick-borne flaviviruses (TBFV) are sustained in nature through cycling between mammalian and tick hosts. In this study, we
used African green monkey kidney cells (Vero) and Ixodes scapularis tick cells (ISE6) to compare virus-induced changes in
mammalian and arthropod cells. Using confocal microscopy, transmission electron microscopy (TEM), and electron
tomography (ET), we examined viral protein distribution and the ultrastructural changes that occur during TBFV infection.
Within host cells, flaviviruses cause complex rearrangement of cellular membranes for the purpose of virus replication. Virus
infection was accompanied by a marked expansion in endoplasmic reticulum (ER) staining and markers for TBFV replication
were localized mainly to the ER in both cell lines. TEM of Vero cells showed membrane-bound vesicles enclosed in a network
of dilated, anastomosing ER cisternae. Virions were seen within the ER and were sometimes in paracrystalline arrays. Tubular
structures or elongated vesicles were occasionally noted. In acutely and persistently infected ISE6 cells, membrane
proliferation and vesicles were also noted; however, the extent of membrane expansion and the abundance of vesicles were
lower and no viral particles were observed. Tubular profiles were far more p
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