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a versatile method for cell-specific profiling of translated mrnas in drosophila一种通用的方法特异性分析翻译mrna的果蝇
A Versatile Method for Cell-Specific Profiling of
Translated mRNAs in Drosophila
1. 1. 2. 1 3,4
Amanda Thomas , Pei-Jung Lee , Justin E. Dalton , Krystle J. Nomie , Loredana Stoica ,
3,4,5 6 6 2
Mauro Costa-Mattioli , Peter Chang , Sergey Nuzhdin , Michelle N. Arbeitman *,
Herman A. Dierick1,3,5,7*
1 Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America, 2 Department of Biomedical Sciences, College of
Medicine, Florida State University, Tallahassee, Florida, United States of America, 3 Department of Neuroscience, Baylor College of Medicine, Houston, Texas, United States
of America, 4 Department of Molecular Cellular Biology, Baylor College of Medicine, Houston, Texas, United States of America, 5 Program in Developmental Biology,
Baylor College of Medicine, Houston, Texas, United States of America, 6 Section of Molecular and Computational Biology, Department of Biological Sciences, University of
Southern California, Los Angeles, California, United States of America, 7 Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, United
States of America
Abstract
In Drosophila melanogaster few methods exist to perform rapid cell-type or tissue-specific expression profiling. A translating
ribosome affinity purification (TRAP) method to profile actively translated mRNAs has been developed for use in a number
of multicellular organisms although it has only been implemented to examine limited sets of cell- or tissue-types in these
organisms. We have adapted the TRAP method for use in the versatile GAL4/UAS system of Dr
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