不同居属地半夏核苷类有效成分比较探究.doc

不同居属地半夏核苷类有效成分比较探究[摘要] 目的：对全国不同居群半夏进行初步的质量评价, 为充分利用和开发半夏资源提供科学依据。方法：收集不同居群的半夏品种，以其有效成分（核苷类成分）含量为评价指标，进行品种质量评价，以遴选出优良半夏品种。总核苷类成分含量（以鸟苷计）采用高效液相色谱法（HPLC）测定。结果：不同居群半夏药材的总核苷类成分存在显著性差异，江苏泰半夏的核苷类成分含量显著高于其他居群。结论：我国半夏种质参差不齐，要培育出优质半夏，需要通过各种人工方法对品质优良的半夏进行品种选育。 [关键词] 半夏；核苷；含量比较；品种选育 [中图分类号] R28[文献标识码]A [文章编号]1673-7210（2010）11（a）-023-03 Comparative study on active ingredients of tubers of Pinellia ternata (Thunb.) Breit. from different populations DAI Xiaobin1, TAN Xianhe1, WU Hao1, WANG Kangcai2, ZHANG Yu1 (1.Department of Pharmacy, Nanjing University Of Traditional Chinese Medicine, Nanjing 210046, China; 2.Nanjing Agricultural University, Nanjing 210000, China) [Abstract] Objective: To evaluate the quality of Rhizoma pinelliae from different populations and provide the scientific basis for using and exploiting resources of Rhizoma pinelliae fully. Methods: To collect Rhizoma pinelliae from different populations, chose their active ingredient (nucleotide composition) content as the indicators for the quality evaluation, selected the excellent breed from them. Furthermore，adopt the method of High-Performance Liquid Chromatography (HPLC) for the content of the nucleotide composition (with guanosine) measurement. Results: The content of nucleotide composition from different populations were significantly different. Rhizoma pinelliae from Taizhou city was much higher than other populations on the content of nucleotide composition. Conclusion: In our country, the quality of Rhizoma pinelliae from different populations is varied. In order to produce high-quality Rhizoma pinelliae, it′s necessary to create the breed of Rhizoma pinelliae which gathers various advantages through some artificial methods. [Key words] Rhizoma pinelliae; Nucleoside composition; Content; Breed 半夏为天南星科植物半夏Pinellia ternata （Thunb.） Breit.的干燥块茎，是我国传统的大宗药材，其性温味辛，有毒，具有燥湿化痰、降逆止呕、消痞散结之功能[1]，是历代医家治疗疾病的要药。半夏块茎的质量是半夏药材品质及质量控制研究的关键，半夏块茎为植物的贮藏器官，含多种药效成分，目前评价半夏质量尚未有统一的成分指标[2]，但以鸟苷和总生物碱作为半夏评价指标成分已