发育毒性体外筛选模型的研究现状和进展-中国食品卫生杂志.PDF

中国食品卫生杂志 　　　 　　　　　 — 152 — 2009 年第 21 卷第 2 期 CHINESE JOURNAL OF FOOD HYGIENE 综述 发育毒性体外筛选模型的研究现状和进展 于 　洲 　徐海滨 ( 中国疾病预防控制中心营养与食品安全所 ,北京 　10002 1) 摘 　要 :利用发育毒性体外评价模型开展相关安全性评价 ,可避免人体或动物实验耗时、费力等缺点 ,适用于大规 模体外高通量筛选 。目前常用的体外评价模型中 ,胚胎干细胞实验 、大鼠全胚胎培养以及体外胚胎肢芽培养实验 因与体内实验结果一致性较强 , 已得到广泛的认可和推荐 。但与一般毒理学体外试验发展相比 , 由于发育毒性受 试物在体内消化代谢路线路径的差异 ,使得发育毒性试验的筛选实验的发展和完善受到阻碍 , 因此合理的利用新 技术 、新方法 ,提高筛选体系的合理性 、系统性成为未来体外评价模型的方向。 关键词 :发育生物学 ;毒性试验 ;实验室技术和方法 ;动物替代试验 Progress in Screening Tests of Developmental Toxicity in Vitro YU Zhou , XU Haibin (National Institute for Nutrition and Food Safety , Chinese CDC , Beij ing 10002 1 , China) Abstract : The safety assessment were carried out with developmental toxicity evaluation model applied to high throughput screening in large scale in vitro , which could avoid the shortcomings of human and animal experiments. In the current evaluation models , the embryonic stem cell test ( EST) , the whole embryo culture (WEC) and the micro mass culture (MM) were widely approved and recommended because they could have the high prediction values between in vitro and vivo tests. The development and advance of screening tests of developmental toxicity were hindered for the differences in vivo routes of chemical substances in comparison with general toxicity tests. So enhancing rationality and systematization of screening system using the application of the new technology and method would be the direction of evaluation model in vitro . Key word : Developmental Biology ;Toxicity tests ;Laboratory Techn