further characterisation of the molecular signature of quiescent and activated mouse muscle satellite cells进一步描述静止的分子签名和激活鼠标肌肉卫星细胞.pdfVIP

Further Characterisation of the Molecular Signature of Quiescent and Activated Mouse Muscle Satellite Cells Viola F. Gnocchi, Robert B. White, Yusuke Ono, Juliet A. Ellis, Peter S. Zammit* Randall Division of Cell and Molecular Biophysics, King’s College London, Guy’s Campus, London, United Kingdom Abstract Satellite cells are the resident stem cells of adult skeletal muscle. To date though, there is a paucity of native markers that can be used to easily identify quiescent satellite cells, with Pax7 probably being the best that is currently available. Here we have further characterized a number of recently described satellite cell markers, and also describe novel ones. Caveolin-1, integrin a7 and the calcitonin receptor proved reliable markers for quiescent satellite cells, being expressed by all satellite cells identified with Pax7. These three markers remained expressed as satellite cells were activated and underwent proliferation. The nuclear envelope proteins lamin A/C and emerin, mutations in which underlie Emery-Dreifuss muscular dystrophy, were also expressed in both quiescent and proliferating satellite cells. Conversely, Jagged-1, a Notch ligand, was not expressed in quiescent satellite cells but was induced upon activation. These findings further contribute to defining the molecular signature of muscle satellite cells. Citation: Gnocchi VF, White RB, Ono Y, Ellis JA, Zammit PS (2009) Further Characterisation of the Molecular Signature of Quiescent and Activated Mouse Muscle Satellite Cells. PLoS ONE 4(4): e5205. doi:10.1371/journal.pone.0005205 Editor: Gianni Parise, McMaster University, Canada Received January 9, 2009; Accepted March 12, 2009; Published April 16, 2009 Copyright: 2009 Gnocchi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which pe