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funduscopy in adult zebrafish and its application to isolate mutant strains with ocular defects眼底镜检查在成年斑马鱼及其应用隔离与眼部缺陷突变株
Funduscopy in Adult Zebrafish and Its Application to
Isolate Mutant Strains with Ocular Defects
1¤ 2 3 3 1
Markus Tschopp , Masanari Takamiya , Kara L. Cerveny , Gaia Gestri , Oliver Biehlmaier , Stephen W.
3 ¨ 2 1
Wilson , Uwe Strahle , Stephan C. F. Neuhauss *
1 Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse, Zurich, Switzerland, 2 Institute of Toxicology and Genetics, Karlsruhe Institute of
Technology, Karlsruhe, Germany, 3 Department of Cell and Developmental Biology, University College London, London, United Kingdom
Abstract
Funduscopy is one of the most commonly used diagnostic tools in the ophthalmic practice, allowing for a ready assessment
of pathological changes in the retinal vasculature and the outer retina. This non-invasive technique has so far been rarely
used in animal model for ophthalmic diseases, albeit its potential as a screening assay in genetic screens. The zebrafish
(Danio rerio) is well suited for such genetic screens for ocular alterations. Therefore we developed funduscopy in adult
zebrafish and employed it as a screening tool to find alterations in the anterior segment and the fundus of the eye of
genetically modified adult animals. A stereomicroscope with coaxial reflected light illumination was used to obtain fundus
color images of the zebrafish. In order to find lens and retinal alterations, a pilot screen of 299 families of the F3 generation
of ENU-treated adult zebrafish was carried out. Images of the fundus of the eye and the anterior segment can be rapidly
obtained and be used to identify alterations in genetically modified animals. A number of putative mutants with
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