有哪些信誉好的足球投注网站genes that influence swarming motility and biofilm formation in variovorax paradoxus eps基因影响聚集在variovorax脉eps能动性和生物膜的形成.pdfVIP
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genes that influence swarming motility and biofilm formation in variovorax paradoxus eps基因影响聚集在variovorax脉eps能动性和生物膜的形成
Genes That Influence Swarming Motility and Biofilm
Formation in Variovorax paradoxus EPS
1 2 2 2 1
Michael J. Pehl , William David Jamieson , Karen Kong , Jessica L. Forbester , Richard J. Fredendall ,
1,3 1,4 1,5 1
Glenn A. Gregory , Jacob E. McFarland , Jessica M. Healy , Paul M. Orwin *
1 Department of Biology, California State University San Bernardino, San Bernardino, California, United States of America, 2 Department of Biology and Biochemistry,
University of Bath, Bath, United Kingdom, 3 Loma Linda University School of Dentistry, Loma Linda, California, United States of America, 4 UCSF School of Pharmacy,
University of California San Francisco, San Francisco, California, United States of America, 5 UCD Department of Public Health Sciences 5215 VM3A, Davis, California, United
States of America
Abstract
Background: Variovorax paradoxus is an aerobic soil bacterium associated with important biodegradative processes in
nature. We use V. paradoxus EPS to study multicellular behaviors on surfaces.
Methodology: We recovered flanking sequence from 123 clones in a Tn5 mutant library, with insertions in 29 different
genes, selected based on observed surface behavior phenotypes. We identified three genes, Varpa_4665, Varpa_4680, and
Varpa_5900, for further examination. These genes were cloned into pBBR1MCS2 and used to complement the insertion
mutants. We also analyzed expression of Varpa_4680 and Varpa_5900 under different growth conditions by qPCR.
Results: The 29 genes we identified had diverse predicted functions, many in exopolysaccharid
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