genetic and molecular analysis of wild-derived arrhythmic mice遗传和分子分析wild-derived心律不齐的老鼠.pdfVIP

Genetic and Molecular Analysis of Wild-Derived Arrhythmic Mice 1 1,2 3 1 4,5 Tsuyoshi Watanabe , Tohru Suzuki , Akira Ishikawa , Yuki Yokota , Hiroki R. Ueda , Rikuhiro G. 4 6 1 1 1 1 1 Yamada , Hajime Tei , Saki Imai , Shigeru Tomida , Junya Kobayashi , Emiko Naito , Shinobu Yasuo , 1 3 1 1 Nobuhiro Nakao , Takao Namikawa , Takashi Yoshimura , Shizufumi Ebihara * 1 Division of Biomodeling, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan, 2 Department of Infectious Disease, Hamamatsu University School of Medicine, Hamamatsu, Japan, 3 Division of Applied Genetics and Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan, 4 Laboratory for Systems Biology, Center for Developmental Biology, RIKEN, Hyogo, Japan, 5 Functional Genomics Subunit, Center for Developmental Biology, RIKEN, Hyogo, Japan, 6 Research Group of Chronogenomics, Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan Abstract A new circadian variant was isolated by screening the intercross offspring of wild-caught mice (Mus musculus castaneus). This variant was characterized by an initial maintenance of damped oscillations and subsequent loss of rhythmicity after being transferred from light-dark (LD) cycles to constant darkness (DD). To map the genes responsible for the persistence of rhythmicity (circadian ratio) and the length of free-running period (t), quantitative trait locus (QTL) analysis was performed using F2 mice obtained from