genetic diversity and microevolution of burkholderia pseudomallei in the environment遗传多样性和洋葱举办微进化的环境.pdfVIP

Genetic Diversity and Microevolution of Burkholderia pseudomallei in the Environment 1. 1. 1 1 Narisara Chantratita , Vanaporn Wuthiekanun , Direk Limmathurotsakul , Mongkol Vesaratchavest , 1 1 1 2 1,3 Aunchalee Thanwisai , Premjit Amornchai , Sarinna Tumapa , Edward J. Feil , Nicholas P. Day , Sharon J. Peacock1,3* 1 Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, 2 Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom, 3 Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom Abstract Background: The soil dwelling Gram-negative pathogen Burkholderia pseudomallei is the cause of melioidosis. The diversity and population structure of this organism in the environment is poorly defined. Methods and Findings: We undertook a study of B. pseudomallei in soil sampled from 100 equally spaced points within 2 237.5 m of disused land in northeast Thailand. B. pseudomallei was present on direct culture of 77/100 sampling points. Genotyping of 200 primary plate colonies from three independent sampling points was performed using a combination of pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Twelve PFGE types and nine sequence types (STs) were identified, the majority of which were present at only a single sampling point. Two sampling points contained four