genome-wide linkage analysis of global gene expression in loin muscle tissue identifies candidate genes in pigs全基因组关联分析的全球基因表达在腰部肌肉组织识别候选基因在猪身上.pdfVIP

Genome-Wide Linkage Analysis of Global Gene Expression in Loin Muscle Tissue Identifies Candidate Genes in Pigs 1,2 1 3 1 Juan Pedro Steibel , Ronald O. Bates , Guilherme J. M. Rosa , Robert J. Tempelman , Valencia D. 1 1 1 1 1,4 Rilington , Ashok Ragavendran , Nancy E. Raney , Antonio Marcos Ramos , Fernando F. Cardoso , 1 1 David B. Edwards , Catherine W. Ernst * 1 Department of Animal Science, Michigan State University, East Lansing, Michigan, United States of America, 2 Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan, United States of America, 3 Department of Animal Sciences, University of Wisconsin, Madison, United States of America, 4 Embrapa Southern Region Animal Husbandry, Rio Grande do Sul, Brazil Abstract Background: Nearly 6,000 QTL have been reported for 588 different traits in pigs, more than in any other livestock species. However, this effort has translated into only a few confirmed causative variants. A powerful strategy for revealing candidate genes involves expression QTL (eQTL) mapping, where the mRNA abundance of a set of transcripts is used as the response variable for a QTL scan. Methodology/Principal Findings: We utilized a whole genome expression microarray and an F2 pig resource population to conduct a global eQTL analysis in loin muscle tissue, and compared results to previously inferred phenotypic QTL (pQTL) from the same experimental cross. We found 62 unique eQTL (FDR ,10%) and identified 3 gene networks enriched with gen