genome-wide requirements for resistance to functionally distinct dna-damaging agents全基因组抵抗功能要求不同的dna有害.pdfVIP

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genome-wide requirements for resistance to functionally distinct dna-damaging agents全基因组抵抗功能要求不同的dna有害.pdf

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genome-wide requirements for resistance to functionally distinct dna-damaging agents全基因组抵抗功能要求不同的dna有害

Genome-Wide Requirements for Resistance to Functionally Distinct DNA-Damaging Agents 1[ 2[ 2 3,4 5 4,6 William Lee , Robert P. St.Onge , Michael Proctor , Patrick Flaherty , Michael I. Jordan , Adam P. Arkin , Ronald W. Davis1,2 2 2* , Corey Nislow , Guri Giaever 1 Department of Genetics, Stanford University School of Medicine, Stanford, California, United States of America, 2 Department of Biochemistry, Stanford University School of Medicine, Stanford Genome Technology Center, Palo Alto, California, United States of America, 3 Department of Electrical Engineering and Computer Science, University of California, Berkeley, California, United States of America, 4 Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, 5 Division of Computer Science, Department of Statistics, University of California, Berkeley, California, United States of America, 6 Howard Hughes Medical Institute, Department of Bioengineering, University of California, Berkeley, California, United States of America The mechanistic and therapeutic differences in the cellular response to DNA-damaging compounds are not completely understood, despite intense study. To expand our knowledge of DNA damage, we assayed the effects of 12 closely related DNA-damaging agents on the complete pool of ;4,700 barcoded homozygous deletion strains of Saccharomyces cerevisiae. In our protocol, deletion strains are pooled together and grown competitively in the presence of compound. Relative strain sensitivity is determined by hybridization of PCR-amplified barcodes to