genomic hiv rna induces innate immune responses through rig-i-dependent sensing of secondary-structured rna艾滋病毒基因组rna诱导通过rig-i-dependent传感secondary-structured rna的先天免疫反应.pdfVIP

Genomic HIV RNA Induces Innate Immune Responses through RIG-I-Dependent Sensing of Secondary- Structured RNA 1 1 2 1 1 Randi K. Berg , Jesper Melchjorsen , Johanna Rintahaka , Elisabeth Diget , Stine Søby , Kristy A. 3 4 2 1 1 Horan , Robert J. Gorelick , Sampsa Matikainen , Carsten S. Larsen , Lars Ostergaard , Søren R. 3 1 Paludan , Trine H. Mogensen * 1 Department of Infectious Diseases, Aarhus University Hospital - Skejby, Aarhus, Denmark, 2 Unit of Excellence for Immunotoxicology, Finnish Institute of Occupational Health, Helsinki, Finland, 3 Department of Biomedicine, University of Aarhus, Aarhus, Denmark, 4 AIDS and Cancer Virus Program, SAIC-Frederick, Inc., National Cancer Institute-Frederick, Frederick, Maryland, United States of America Abstract Background: Innate immune responses have recently been appreciated to play an important role in the pathogenesis of HIV infection. Whereas inadequate innate immune sensing of HIV during acute infection may contribute to failure to control and eradicate infection, persistent inflammatory responses later during infection contribute in driving chronic immune activation and development of immunodeficiency. However, knowledge on specific HIV PAMPs and cellular PRRs responsible for inducing innate immune responses remains sparse. Methods/Principal Findings: Here we demonstrate a major role for RIG-I and the adaptor protein MAVS in induction of innate immune responses to HIV genomic RNA. We found that secondary structured HIV-derived RNAs induced a response similar to genomic RNA. In prim