genomic instability is associated with natural life span variation in saccharomyces cerevisiae基因组不稳定性与酿酒酵母的自然寿命的变化.pdfVIP

Genomic Instability Is Associated with Natural Life Span Variation in Saccharomyces cerevisiae 1¤ 1 2 Hong Qin *, Meng Lu , David S. Goldfarb 1 Center for Aging and Development Biology, Department of Biology, University of Rochester, Rochester, New York, United States of America, 2 Department of Biology, University of Rochester, Rochester, New York, United States of America Abstract Increasing genomic instability is associated with aging in eukaryotes, but the connection between genomic instability and natural variation in life span is unknown. We have quantified chronological life span and loss-of-heterozygosity (LOH) in 11 natural isolates of Saccharomyces cerevisiae. We show that genomic instability increases and mitotic asymmetry breaks down during chronological aging. The age-dependent increase of genomic instability generally lags behind the drop of viability and this delay accounts for ,50% of the observed natural variation of replicative life span in these yeast isolates. We conclude that the abilities of yeast strains to tolerate genomic instability co-vary with their replicative life spans. To the best of our knowledge, this is the first quantitative evidence that demonstrates a link between genomic instability and natural variation in life span. Citation: Qin H, Lu M, Goldfarb DS (2008) Genomic Instability Is Associated with Natural Life Span Variation in Saccharomyces cerevisiae. PLoS ONE 3(7): e2670. doi:10.1371/journal.pone.0002670 Editor: Dana Davis, University of Minnesota, United States of America Received March 19, 2008; Accepted June 13, 2008; Published July 16, 2008 Copyright: 2008 Qin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reprod