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2017-09-01 发布于上海
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growth of mouse oocytes to maturity from premeiotic germ cells in vitro增长的成熟小鼠卵母细胞减数分裂前的生殖细胞体外.pdf

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growth of mouse oocytes to maturity from premeiotic germ cells in vitro增长的成熟小鼠卵母细胞减数分裂前的生殖细胞体外

Growth of Mouse Oocytes to Maturity from Premeiotic Germ Cells In Vitro 1 1 1 1 1 1 Zhi-Peng Zhang , Gui-Jin Liang , Xi-Feng Zhang , Guo-Liang Zhang , Hu-He Chao , Lan Li , Xiao- 1 1 1 2 1,3 4 Feng Sun , Ling-Jiang Min , Qing-Jie Pan , Qing-Hua Shi , Qing-Yuan Sun , Massimo De Felici , Wei Shen1* 1 Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao Agricultural University, Qingdao, China, 2 Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China, 3 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, 4 Department of Biomedicine and Prevention, University of Rome ‘Tor Vergata’, Rome, Italy Abstract In the present study, we established an in vitro culture system suitable for generating fertilizable oocytes from premeiotic mouse female germ cells. These results were achieved after first establishing an in vitro culture system allowing immature oocytes from 12–14 day- old mice to reach meiotic maturation through culture onto preantral granulosa cell (PAGC) monolayers in the presence of Activin A (ActA). To generate mature oocytes from premeiotic germ cells, pieces of ovaries from 12.5 days post coitum (dpc) embryos were cultured in medium supplemented with ActA for 28 days and the oocytes formed within the explants were isolated and cocultured onto PAGC monolayers in the presence of ActA for 6–7 days. The oocytes were then subjected to a final meiotic maturation assay to