high throughput sequencing and proteomics to identify immunogenic proteins of a new pathogen the dirty genome approach高通量测序和蛋白质组学鉴定免疫原性蛋白的新病原体的基因组的方法.pdfVIP

High Throughput Sequencing and Proteomics to Identify Immunogenic Proteins of a New Pathogen: The Dirty Genome Approach 1. 1. 1. 1 2,3 Gilbert Greub *, Carole Kebbi-Beghdadi , Claire Bertelli , Franc¸ois Collyn , Beat M. Riederer , 1 1 4 Camille Yersin , Antony Croxatto , Didier Raoult 1 Center for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, University Hospital Center, University of Lausanne, Lausanne, Switzerland, 2 Department of Cellular Biology and Morphology, University of Lausanne, Lausanne, Switzerland, 3 Proteomics Unit, Department of Psychiatric Neurosciences, Cery, Prilly-Lausanne, ´ ´ ´ ´ ´ ´ Switzerland, 4 Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, Marseille, France Abstract Background: With the availability of new generation sequencing technologies, bacterial genome projects have undergone a major boost. Still, chromosome completion needs a costly and time-consuming gap closure, especially when containing highly repetitive elements. However, incomplete genome data may be sufficiently informative to derive the pursued information. For emerging pathogens, i.e. newly identified pathogens, lack of release of genome data during gap closure stage is clearly medically counterproductive. Methods/Principal Findings: We thus investigated the feasibility of a dirty genome approach, i.e. the release of unfinished genome sequences to develop serological diagnostic tools. We showed that almost the whole genome sequence of the emerging pathogen Parachlamydia