highly pathogenic influenza a(h5n1) virus survival in complex artificial aquatic biotopes高致病性流感a(h5n1)型病毒生存在复杂人工水生生物栖地.pdfVIP

Highly Pathogenic Influenza A(H5N1) Virus Survival in Complex Artificial Aquatic Biotopes 1 ´ 1 2 1 Viseth Srey Horm , Ramona A. Gutierrez , John M. Nicholls , Philippe Buchy * ´ 1Virology Unit, Institut Pasteur du Cambodge, Reseau International des Instituts Pasteur, Phnom Penh, Cambodia, 2 Department of Pathology, University of Hong Kong, Pokfulam, Hong Kong, Hong Kong SAR Abstract Background: Very little is known regarding the persistence of Highly Pathogenic Avian Influenza (HPAI) H5N1 viruses in aquatic environments in tropical countries, although environmental materials have been suggested to play a role as reservoirs and sources of transmission for H5N1 viruses. Methodology/Principal Findings: The survival of HPAI H5N1 viruses in experimental aquatic biotopes (water, mud, aquatic flora and fauna) relevant to field conditions in Cambodia was investigated. Artificial aquatic biotopes, including simple ones containing only mud and water, and complex biotopes involving the presence of aquatic flora and fauna, were set up. They were experimentally contaminated with H5N1 virus. The persistence of HPAI H5N1 virus (local avian and human isolates) was determined by virus isolation in embryonated chicken eggs and by real-time reverse-polymerase chain reaction. Persistence of infectious virus did not exceed 4 days, and was only identified in rain water. No infectious virus particles were detected in pond and lake water or mud even when high inoculum doses were used. However, viral RNA persisted up to 20 days in rain water and 7 days in pond or lake