high-throughput 13-parameter immunophenotyping identifies shifts in the circulating t-cell compartment following reperfusion in patients with acute myocardial infarction高通量13-parameter immunophenotyping标识的变化循环t细胞在急性心肌梗死患者再灌注后车厢.pdfVIP

High-Throughput 13-Parameter Immunophenotyping Identifies Shifts in the Circulating T-Cell Compartment Following Reperfusion in Patients with Acute Myocardial Infarction 1 5 3 1 1 4 Jedrzej Hoffmann , Karel Fiser , Jolanta Weaver , Ian Dimmick , Monika Loeher , Hanspeter Pircher , 2 1 1 2 Carmen Martin-Ruiz , Murugapathy Veerasamy , Bernard Keavney , Thomas von Zglinicki , Ioakim Spyridopoulos1* 1 Institute of Genetic Medicine, Newcastle University, Newcastle, United Kingdom, 2 Institute of Ageing and Health, Newcastle University, Newcastle, United Kingdom, 3 Institute of Cellular Medicine, Newcastle University, Newcastle, United Kingdom, 4 Department of Immunology, Institute of Medical Microbiology and Hygiene, Freiburg University, Freiburg, Germany, 5 CLIP – Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Medical School, Charles University Prague, Prague, Czech Republic Abstract Rationale: With the advent of primary PCI (PPCI), reperfusion is achieved in almost all patients presenting with acute myocardial infarction. However, despite multiple trials, reperfusion injury has not been successfully dealt with so far. In mouse models, CD4+ T lymphocytes (T cells) have been shown to be crucial instigators of reperfusion injury. Objective: Our goal was to investigate the role of CD4+ T cells during myocardial reperfusion following PPCI by developing a protocol for high-throughput multiplexed flow cytometric analysis and multivariate flow clustering. Methods and Results: 13-parameter immunophenotyping and hierarchical cluster