human alpha defensin 5 expression in the human kidney and urinary tract人类αdefensin 5表达人类的肾脏和泌尿道.pdfVIP

Human Alpha Defensin 5 Expression in the Human Kidney and Urinary Tract 1,2. 2,3. 4 5 3 John David Spencer , David S. Hains , Edith Porter , Charles L. Bevins , Julianne DiRosario , Brian 1,2 3 2,3 . Becknell , Huanyu Wang , Andrew L. Schwaderer * 1 Pediatric Nephrology Fellowship Program, Nationwide Children’s Hospital, Columbus, Ohio, United States of America, 2 Division of Nephrology, Department of Pediatrics, Nationwide Children’s Hospital, Columbus, Ohio, United States of America, 3 Center for Clinical and Translational Research, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, United States of America, 4 Department of Biological Sciences, California State University Los Angeles, Los Angeles, California, United States of America, 5 Department of Microbiology and Immunology, School of Medicine, University of California Davis, Davis, California, United States of America Abstract Background: The mechanisms that maintain sterility in the urinary tract are incompletely understood. Recent studies have implicated the importance of antimicrobial peptides (AMP) in protecting the urinary tract from infection. Here, we characterize the expression and relevance of the AMP human alpha-defensin 5 (HD5) in the human kidney and urinary tract in normal and infected subjects. Methodology/Principal Findings: Using RNA isolated from human kidney, ureter, and bladder tissue, we performed quantitative real-time PCR to show that DEFA5, the gene encoding HD5, is constitutively expressed throughout the u