血必净对大鼠mods模型的外周血tnf-αil-1α-中国实验动物学报.doc

尊敬的编辑，您好！非常感谢您对本文提出的宝贵意见，我已经遵照您的要求进行文章的补充修改，但在大鼠的心跳及呼吸这一点当时在实验时因为条件的制约，我们实验室对大型实验动物可以进行监控，但是对于大鼠的监控因为难度较大，所以没有进行监控，所以对你提出的心跳和呼吸这两个数据没有采集。希望您能酌情修改，能使文章在逻辑上更严谨更具有条理性。再次感谢您在百忙之中对我的文章的审阅，祝安！ (血必净对大鼠多脏器功能障碍综合征炎性因子的影响 陆　璐1，冯小明1，王红义1，牛廷献1，万东君3，王春雨2，罗晓红2 * (兰州军区兰州总医院　1. 动物实验科，2. 内分泌科，3. 干部病房 兰州　730050) 【摘要MODS）炎性因子的影响。方法 将90只Wistar大鼠随机分为对照组、模型组与XBJ组。尾静脉注射内毒素（LPS）（15 μg / kg）建立MODS模型。观察各组的体征表现，用ELISA方法检测TNF-α、IL-1αTNF-α的表达，同时抑制IL-1α的表达，且TNF-α和IL-1α高表达集中在6h~24h；③ 模型组与XBJ组IL-4的表达量在6h~12h与对照组相比明显升高（P＜0.05）；模型组IL-10在6h~48h与对照组相比明显升高（P＜0.05）；XBJ组IL-10的表达水平在6h与对照组相比明显升高（P＜0.05）。结论　XBJ能降低LPS介导的大鼠MODS的死亡率，可同时促进TNF-α释放和抑制IL-1α、IL-4和IL-10释放，有效改善MODS的反应状态。 【关键词多脏器功能障碍综合征；ultiple Organ Dysfunction Syndrome in Rat LU Lu1,FENG Xiao-ming1, WANG Hong-yi1, NIU Ting-xian1, WAN Dong-jun3, WANG Chun-yu2, LUO Xiao-hong2* (Lanzhou General Hospital of PLA, Department of Animal Experiment, Lanzhou,730050,China) [Abstract] Objective To evaluate the influence of Xuebijing (XBJ) by inflammation cytokines of multiple organ dysfunction syndrome (MODS). Methods 90 rats were randomly separated into 3 groups, the control group and XBJ group were infused 15 μg / kg LPS in vena caudalis, Then the physical sign appearance was observed and collection the serum to determine the expression concentration of TNF-α、IL-1αin each group at different times. Results ① The death rate of rats were the percent of 0, 37, and 20 in the control group，model group and XBJ group, respectively. ② The XBJ enhance the increased amplitude of TNF-α and deduce the increased amplitude of IL-1α by compared with model group, the expression concentration peak of TNF-α and IL-1α is 6h~24h. ③ The expression concentration of IL-4 in model group and XBJ group in model group has significant ascendant compared with control group at 6h~48h; XBJ group has significant have significant ascendant compared with control group at 6h~12h (p0.05).The expression concentration of IL-10 ascendant compared with control group at 6h (p0.05). Conclusion